Figure 2 | Scientific Reports

Figure 2

From: Neutralization of Acidic Intracellular Vesicles by Niclosamide Inhibits Multiple Steps of the Dengue Virus Life Cycle In Vitro

Figure 2

Niclosamide affects both the early and late stages of dengue virus replication. (a) Schematic representation of the time-of-addition experiment. Niclosamide was added to DENV-2-infected cells at the indicated time points and remained present until sample collection. DMSO was used as a control. (b) Infectious virus titres in the supernatants were determined by focus-forming assay. (c and d) Parallel infections were collected for quantification of intracellular and extracellular DENV-2 RNAs by RT-qPCR. (c) Intracellular viral RNA levels (E gene) were normalized to β-actin mRNA. (d) Extracellular viral RNA levels were expressed as mRNA copy numbers per ml (copies/ml). (e) DENV-2 NS3 and E protein levels in lysates of infected cells were determined by Western blot analysis. Different probing is divided by white space. Uncropped images are presented in the supplemental material. CC; cell control. VC; virus control. The data represent the means (±SD) of at least two independent experiments performed in duplicate. **p < 0.01 and ****p < 0.0001 compared to DMSO control.

Back to article page