Figure 3
Photomicrographs showing localization of superoxide anion (NBT staining) and hydrogen peroxide (DAB staining) in tomato seeds. (A) superoxide anion in 12 h unprimed seeds; (B) superoxide anion in 12 h primed seeds; (C) superoxide anion in 24 h unprimed seeds; (D) superoxide anion in 24 h primed seeds. The formation of insoluble blue formazan precipitates in the endosperm of primed seeds indicate the strong accumulation of O2•− as compared to unprimed seeds. (E) hydrogen peroxide in 12 h unprimed seeds; (F) hydrogen peroxide in 12 h primed seeds; (G) hydrogen peroxide in 24 h unprimed seeds; (H) hydrogen peroxide in 24 h primed seeds. Increased brown coloration in outer part of the endosperm of primed seeds shows higher production of H2O2 as compared to unprimed seeds.
