Figure 6
(A–C). Bar graphs depicting the effect of magnetic field treatment on the relative expression of various genes in germinating tomato seeds at 12 h of imbibition. (A) Relative expression of genes involved in the synthesis of H2O2, i.e. AO (Amine oxidase), NOX (NADPH oxidase), SOD1 and SOD9 (Superoxide dismutase 1 and 9), and ArcA2 (receptor for activated C kinase 1/RACK1 homologue)#; scavenging of H2O2, i.e. CAT1 (Catalase 1), APX1 and APX2 (Ascorbate peroxidase 1 and 2), MT1 (Metallothionein 1); signaling of H2O2, i.e. ArcA2 and MT1. (B) Relative expression of isoforms of Metallothionein (MT1, MT3 and MT4)#. (C) Relative expression of ABA-H (ABA 8′-hydroxylase) and GA3ox1 and GA3ox2 (Gibberellic acid 3 oxidase1 and 2). Data represent mean ± S.E. of three biological replicates with two technical replications. GAPDH gene was used as internal control to normalize the expression level. Fold change was calculated using 2−ΔΔCT method that represents the changes in expression levels in the primed seeds as compared to the unprimed ones. Significant difference between treatments is indicated with the p value, according to Student’s t test. ns = non significant. qRT-PCR primers for all genes are provided in Table 2. #ArcA2 gene is involved in synthesis as well as signaling processes of H2O2, while MT1 gene is involved in signaling as well as scavenging processes of H2O2.
