Table 2 Primers used to check the expression of genes involved in hydrogen peroxide production, scavenging and signaling mechanisms.
S.No. | Enzyme/Property | Gene Name | Accession No. | Primer pair | |
|---|---|---|---|---|---|
1. | Cu containing amine oxidase | AO | NM_001310065.1 | F | ATACGGGTTCGGGTTACA |
R | CCATCAGCTGCCACAAATA | ||||
2. | NADPH oxidase | NOX/RBOH1 | NM_001247197.2 | F | GCCGAATTGGAGGAAAGTAT |
R | CAGAGTTGGCTGAGTTCTTT | ||||
3. | Superoxide dismutase Cu-Zn | SOD1 | NM_001311084.1 | F | CCACAAATGGCTGTATGTCAAC |
R | TACCAAGATCACCAGCATGAC | ||||
4. | Superoxide dismutase Mn | SOD9 | NM_001330621 | F | TTTAACGGCGGAGGTCATATT |
R | CAATAGCCCAGCCAAGAGAA | ||||
5. | Receptor for activated C kinase 1 (RACK 1) homologue | ArcA2 | AB022687.1 | F | GTGGCAAGGATGGAGTTATT |
R | AGGACTAAAGCAGAGAGTATGA | ||||
6. | Metallothionein type 2 | MT1 | NM_001247117.2 | F | GTTGAAACAGAGCAGAGATCA |
R | TTCAACAGACAGACACACTAAA | ||||
7. | Metallothionein type 2 | MT3 | NM_001247125.2 | F | GTGGATCTGGCTGCAAGT |
R | GTGCAACACCTTCAACGAT | ||||
8. | Metallothionein type 2 | MT4 | NM_001247362.2 | F | TGGATCTGGCTGCAAGTG |
R | CTCAGCCACTCCATAGTTCTTC | ||||
9. | Catalase | CAT1 | KU933832.1 | F | GTACCCGATTCCTTCTTGTG |
R | TCCCATGATCTGTACCTCTC | ||||
10. | Ascorbate peroxidase | APX1 | NM_001247853.2 | F | ACGATGATATTGTGACACTCTTCCA |
R | AAGCGATGAAACCACAAAAACA | ||||
11. | Ascorbate peroxidase | APX2 | NM_001247702.2 | F | TGGGAGGGTGGTGACATATTTT |
R | TTGAAGTGCATAACTTCCCATCTTT | ||||
12. | ABA 8′- hydroxylase | ABA-H/CYP707A2 | HQ008774.1 | F | CCATCGCGATAACATCACTC |
R | TTCTTGAGCTCCTCTCTGTAT | ||||
13. | Gibberellic acid3 oxidase1 | GA3ox1 | AB010991.1 | F | GATGTGCTGCCTTACAACTA |
R | GGTAGAATCCGTATGTGCTG | ||||
14. | Gibberellic acid3 oxidase2 | GA3ox2 | NM_001246926.3 | F | CGGAGTCCATTCCAGTAATC |
R | GGTTAAGTAATCGGTGCGATA | ||||
15. | Glyceraldehyde 3-phosphate dehydrogenase | GAPDH | NM_001247874.2 | F | GGAGACAATAGACCAAGCATATT |
R | TGAATAATCAAGTCCACCACTC | ||||
