Figure 2
Blood cells, PBPCs and neuroblastoma cells differ in size and acoustic properties. (a) Cell size distributions of different peripheral blood cell preparations and neuroblastoma cells. SH-SY5Y neuroblastoma cells (left), blood MNCs (upper right), PBPC MNCs (middle right), and unprocessed PBPCs (lower right) are shown. PBPC MNC and PBPC samples were from the same donors. The different PBPC donors are indicated by orange: healthy donor, blue: myeloma patients. Different shades of the same colour present different passages (red) or different donors, respectively (blue). Cell numbers are shown as normalized cell counts relative to the total number of cells acquired for each individual sample (n = 3). Counts in the small size range account for dead or apoptotic cells as well as debris in the samples. (b,c) Peripheral blood MNCs and NB cells behave differently in the acoustophoresis chip. MNCs (1 × 106 cells/mL) and NBCs (1 × 103–1 × 106 cells/mL) were evaluated separately. (b) MNCs collected in the side outlet (left) and center outlet (right) when exposed to different acoustic field strengths by increasing voltages on the separation channel. (c) NBCs collected in the side outlet (left) and center outlet (right) using the same voltages as in (b). The fraction of cells collected per outlet is expressed as percent of total collected cells. Data are shown as mean ± SD of 3 independent experiments. BD represents data below the detection limit.
