Figure 1

Changes in pH values and cell numbers in OF during cultivation. Neonatal OF was cultured with breast milk (BM, blue line) or YCFA (red line) media for 48 h in pH-stat fermenters. (A) The pH value was continuously monitored, and each point represents the mean value with the S.E. (n = 15). (B) qPCR was performed to evaluate total cell numbers. The limit of detection was 104 cells/mL or g. Groups without the same letter were statistically different using the Tukey-Kramer test (p < 0.05).