Figure 5
Expression characteristics of H4_GE cells – kinetics of protein amounts. H4 cell lines were incubated with 10 µM tebufenozide for 0 to 5 days. For all samples, expression of housekeeper β-tubulin was used as reference. (A) Control Western blots of H4 cells were treated like the Western blots of the corresponding samples and showed no expression of αSyn, Venus, SV, V1S, or SV2. (B) In all transgene expressing cell lines signal intensity increased over time for inducible transgenes. Full-length Western blots and additional exposures are shown in Supplementary Figs 7, 8, respectively. (C) Quantification of Western blot signal intensity was performed using ImageJ. Signal intensity of transgenes was normalized to maximum. Data points were derived from the blots shown in A and B.
