Figure 6
Increased fluorescence intensity in H4_GE-V1S + SV2 cells upon incubation with DMSO and FeCl3. H4_GE-V1S + SV2 and H4_GE-V cells were incubated with tebufenozide and different concentrations of DMSO and tri-, di-, or monovalent ions for 3 days. (A) Mean cellular Venus fluorescence intensity was increased upon incubation with higher DMSO and FeCl3 concentrations in H4_GE-V1S + SV2 cells (left), but not in H4_GE-V cells (right). Bars show mean of three independent experiments. (B) Fluorescence intensity increased upon incubation with FeCl3 compared to other trivalent (AlCl3, GdCl3), divalent (CuCl2, ZnCl2, MnCl2), or monovalent (NaCl) ions. All cells were co-incubated with 1% DMSO. Data points show mean of 8 to 9 independent experiments; error bars show SEM. *p’ < 0.05; **p’ < 0.01; ***p’ < 0.001 (Student’s t-test, Bonferroni corrected for multi-testing).
