Table 1 Parameters used to run the simulations of the power analyses. All possible combinations of these parameters (60 total) were run.

From: Effect of heritable symbionts on maternally-derived embryo transcripts

Coverage (X)

Coefficient of variation (CV)c

Number of replicatesd

Effect size (log-fold change)e

Alfa (threshold p-value)

20a

0.2

3

1.5

0.05

10b

0.3

4

1.75

 
 

0.4

 

2

 
   

3

 
   

4

 
  1. abased on the Lander/Waterman estimate of coverage for our data; i.e., mean coverage of 23X per replicate (range 19–28 among our replicates).
  2. bbased on our minimum number of reads per replicate (in one treatment) required for a DE test (see text)
  3. cThese values encompass the range of most of the values of Biological Coefficient of Variation (BCV; estimated by edgeR) obtained (i.e., a “Common” value of ~0.31 and a stabilized “Trend” value of ~0.2; see Fig. 2).
  4. dreflects the range of replicates per treatment used in this study
  5. epower was high and stable beyond an effect size of 4 (see Fig. 5). Thus, higher effect sizes are not reported.
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