Figure 4 | Scientific Reports

Figure 4

From: The levels of the NMDA receptor co-agonist D-serine are reduced in the substantia nigra of MPTP-lesioned macaques and in the cerebrospinal fluid of Parkinson’s disease patients

Figure 4

mRNA and protein expression of DDO, DAAO and SR in the brain of parkinsonian monkeys. (a,d,g) DDO, (b,e,h) DAAO and (c,f,i) SR mRNA expression was detected by quantitative RT-PCR in the (a–c) putamen, (d–f) substantia nigra and (g-i) medial frontal gyrus of untreated (control), MPTP- and (MPTP + L-DOPA)-treated monkeys (n = 5 monkeys/treatment). mRNA expression is normalized to the mean of three housekeeping genes and expressed as arbitrary units. (b,h) N.D. indicates that DAAO transcript was not detectable up to 40 cycles. (j) DAAO and (k) SR protein levels were detected by Western blotting in the same putamen and substantia nigra samples used for quantitative RT-PCR. (j) N.D. indicates that DAAO protein was not detectable. Proteins variations are expressed as percentage of the control group. Representative blots of DAAO and SR immunodensity comparing the experimental groups are shown above the graph. Full-length blots are presented in Supplementary Fig. S1. Tubulin was used to normalize for variations in loading and transfer. *p < 0.05, compared to control group (Fisher’s post-hoc). Dots represent the single subjects’ values while bars illustrate the means ± SEM.

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