Table 1 Excitation Energy Transfer from FPs to the carotenoid of OCP and calculated FRET parameters.

From: A genetically encoded fluorescent temperature sensor derived from the photoactive Orange Carotenoid Protein

OCP State

Energy Donor

TagGFP

TagRFP

FP-OCP Complex Configuration

1 – “tight’’

2 – “loose”

1 – “tight

2 – “loose”

OCPO

EET Efficiency E, %

82.7 ± 1.5

25.9 ± 1.6

72.8 ± 1.3

15.5 ± 1.5

Population, %

67.5 ± 2.7

32.5 ± 2.6

51.5 ± 2.4

48.5 ± 2.3

Donor-Acceptor Distance R, Å

44.1

68.2

34.7

54.4

Förster radius R0, Å

57. 3

40.9

OCPR

EET Efficiency E, %

80.2 ± 2.4

22.8 ± 2.2

74.6 ± 2.9

20.0 ± 2.6

Population, %

57.6 ± 3.0

42.4 ± 2.9

56.2 ± 2.8

43.8 ± 2.8

Donor-Acceptor Distance R, Å

43.6

67.5

40.0

60.3

Förster radius R0, Å

55.1

47.9

  1. EET efficiency values E and population fractions were estimated from the fluorescence decay components of FPs (see Fig. 2). Förster radius R0, and Donor-Acceptor distance R were calculated assuming the random orientation of the transition dipoles κ2.
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