Figure 6
YB-1 is essential for SG formation and PAC2 cell viability upon thermal stress. (a) Confocal immunofluorescence analysis of G3BP1 (red) and YB-1 (green) in control (upper panel) and YB-1-silenced PAC2 cells (lower panel) after heat shock treatment (45 minutes at 45 °C). Nuclei were stained with DAPI (blue) and both single and merged images are presented. White arrows indicate SGs; (b) cell viability assay (MTT) in control or YB-1-silenced PAC2 cells at 26 °C or after heat shock (45 minutes at 45 °C). Statistical analysis was performed using 1-way ANOVA followed by Tukey’s multiple comparisons test. Levels of significance are indicated (***p 0.001) (see also Table S1).
