Figure 8

Heat shock, Arsenite, Copper and H2O2 treatment promotes assembly of YB-1 positive aggregates in human HDF fibroblast cells. (a) Representative confocal immunofluorescence images of HDF cells stained with human α-YB-1 C- ter antibody (green). Some YB-1 aggregates are indicated by arrows. (b) Cell viability assay (MTT) in PAC2 and HDF cells after 45 minutes treatment with different doses of H2O2. Statistical analysis was performed using 1-way ANOVA followed by Tukey’s multiple comparisons test. Levels of significance are indicated (***p 0.001) (see also Table S1).