Figure 5

Myelin Basic Protein (MBP) expression. A, Protein expression of MBP was evaluated by immunohystochemistry in each experimental group. CCI group and CCI + laser group were compared to each other and with sham group. Original magnification 400X. Scale bar = 20 μm. B, AEC intensity was calculated by the integrated density of pixels for MBP. Control condition was arbitrarily set as 100% and results are expressed as mean ± S.E.M of 6 rats per group. Results are representative of at least three independent immunohistochemistry evaluations. *P < 0.05 vs sham group and °P < 0.01 vs CCI group. Each experimental point was performed in triplicate. Pictures are representative of fifteen field captured for each experimental point. *P < 0.01 vs sham group; °P < 0.01 vs CCI group.