Figure 3

HIFU pulsing protocol used in the present study. 10 ms long HIFU pulse, 1 Hz pulse repetition frequency, 1% duty cycle and the number of HIFU pulses of 5, 25, 50, 100 or 200 were employed. Simulated peak positive P₊ and negative P₋ pressures at the HIFU focus were 85 and −14 MPa in situ.