Figure 1

Study design of the artificial infection experiment in neonatal mice. Male and female pups from 3 litters were infected 4 days after birth with G. muris cysts. At days 7, 21 and 60 post-infection, corresponding with days 11, 25 and 64 after birth, respectively, 5 pups were selected at random from both the infected and control litters. The pups were euthanized, tissue samples were collected and trophozoites present in the small intestine counted. Faecal cyst counts were performed every 2 days from day 21 p.i. onwards.