Figure 4
On-chip Ca2+ imaging in C2C12 cells. (a) Fluorescence image of C2C12 cells overlaid with the image of the filter-integrated array (false coloured, R1 − R8 and C1 − C8 are the row and column numbers of the array). One PD pixel with one cell right on top (outlined) is squared for data analysis in b. Scale bar, 20 μm. (b) Measured ΔVsig/Vsig0 traces when 100 μM A23187 ionophores (yellow) or only DMSO-premix (black) were added to the cells. The two ΔVsig/Vsig0 traces are from the PD pixels (right below the targeted cells) squared in a and Supplementary Fig. S5g, respectively. (c) Array-collected Vsig0-mapping data after calibrating out the pixel-to-pixel variation. (d) Microscope-collected F0-mapping data. The presented F0 values are averaged from the region of each PD pixel in a. (e) Array-collected ΔVsig/Vsig0-mapping data from the ionophore adding experiment (after calibrating out the pixel-to-pixel variation). (f) Microscope-collected ΔF/F0-mapping data from the ionophore adding experiment. The presented ΔF/F0 values are averaged from the region of each PD pixel in a. (g) Array-collected ΔVsig/Vsig0-mapping data from the control experiment (after calibrating out the pixel-to-pixel variation). The absolute values of the presented data are in the same scale of e. (h) Microscope-collected ΔF/F0-mapping data from the control experiment. The absolute values of the presented data are in the same scale of (f).
