Figure 2

TGCT specific miRNA expression in TGCT cells after cisplatin treatment. The expression of ten miRNAs was analysed by qPCR in both 833 K and NT2-D1 cells after 24-hour incubation with cisplatin (6 μM). Both in 833 K (a) and NT2-D1 cells (b), the expressions of miR-302a-3p, miR-302b-3p and miR-302c-3p were significantly downregulated while the downregulation of miR-302d-3p was only significant in NT2-D1 cells. The miR-302a-5p, miR-200c-3p and miR-367-3p showed no significant changes, and the expression of the miR-371-373 cluster was not detected in either cell line. Relative fold change of miRNA expression was determined using the equation RQ = 2^−ΔΔCT. t-test: Control vs Cisplatin (6 μM), mean ± SD (calculated from three independent experiments), statistical significance p < 0.05. The designation on the x-axis indicates the miRNA.