Figure 4
From: Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
No induction of DNA damage by ELV obtained from mitochondria depleted (rho 0) cells. (A) Morphology of rho 0 (ρ0) cells and their parental (HDFn) cells. (B) Representative image of MitoTracker CMXRos retention (red) in HDFn cells and ρ0 cells, counterstained with DAPI (blue). (C) PCR amplification of mitochondrial ND1 (upper panel) and ND5 (middle panel), internal control beta-actin (lower panel) of HDFn cells and ρ0 cells. Full images of gels are present in Supplementary Fig. S3. (D) A schematic view of the experimental protocol. (E) Detection of CD9 and TGS101 proteins in the ELV in medium from control ρ0 cells and 4 Gy exposed ρ0 cells. Full-length blots are present in Supplementary Fig. S2. (F) Representative image of PKH67 labeled ELV (green) derived from control ρ0 cells and 4 Gy exposed ρ0 cells, counterstained with DAPI (blue). (G) The frequency of DNA damage foci in un-treated cells (control), cells treated with ρ0 CCCM ELV and cells treated with ρ0 ICCM ELV. Values are represented as mean ± standard error, with significant differences between indicated groups (*) calculated by Chi-square test (p < 0.01).
