Figure 7
From: Radiation-Induced Bystander Effect is Mediated by Mitochondrial DNA in Exosome-Like Vesicles
Induction of DNA damage by amplified mouse mtDNA in ELV. (A) PCR amplification of mitochondrial mND1 (upper panel) and nuclear mSLCO2B1 (lower panel) in control (0 Gy) mouse serum ELV (n = 3; #1 - #3) and irradiated (4 Gy) mouse serum ELV (n = 3; #1 - #3). Full images of gels are present in Supplementary Fig. S3. (B) Quantification of mitochondrial DNA (mtDNA; mND1 and mND5) and nuclear DNA (nDNA; mSLCO2B1 and mSERPINA1) by RT-PCR in control (0 Gy) mouse serum ELV and irradiated mouse serum ELV. Values are represented as mean ± standard error, with significant differences between indicated groups (*) calculated by Chi-square test (p < 0.01). (C) Representative image of PKH67 labeled ELV (green) derived from control mouse serum ELV with amplified mtDNA (+ND1 and +ND5) and amplified nDNA (+SLCO2B1 and + SERPINA1) up-taken by treated m5S cells, counterstained with DAPI (blue). (D) The frequency of DNA damage foci in un-treated cells (control), control mouse serum ELV + ND1, control mouse serum ELV + ND5, control mouse serum ELV + SLCO2B1, and control mouse serum ELV + SERPINA1 treated cells. Values are represented as mean ± standard error, with significant differences between indicated groups (*) calculated by Chi-square test (p < 0.01).
