Figure 5
From: NFATc3 controls tumour growth by regulating proliferation and migration of human astroglioma cells
Gene expression regulation by NFATc3 and NFATc1 silencing in glioma cells. KD-Sc, KD-c1 and KD-c3 U251 cells were non stimulated (ns) or 4 h treated with 1 μM Io in combination with 20 ng/mL PMA (PIo). (A to I) RCAN1-4; COX-2; TNFα; CXCR3; GM-CSF; IL-2; CCL2; IL-8 and VEGF mRNAs were quantified and normalized to the expression of TFRC and GAPDH as endogenous control. Levels are presented as the fold expression above non-stimulated cells (ns that was given a value of 1 in arbitrary units). Values are the mean ± SD of triplicate RT-PCR determinations for each condition. ***P < 0.001; **P < 0.01; *P < 0.05 (ANOVA) with respect to PIo stimulated KD-Sc cells.
