Figure 4

Evaluation of the cytokines in the plasma. (A) The plasma cytokine concentrations of IFN-γ, IL-10, IL-17A, IL-1β, IL-6, and TNF-α were quantified by enzyme-linked immunosorbent assay in the following groups: control group (control) and PF group (PF) just before infection (0 h); and infected with or without micafungin administration, in both PF and control groups at 12 h after the infection (four mice each). All the six cytokines at 12 h after the intraperitoneal inoculation of C. albicans tended to be suppressed with micafungin treatment in both PF and control groups. (B) The plasma TNF-α concentrations of LPS-exposed mice instead of C. albicans inoculation were quantified by enzyme-linked immunosorbent assay, and no significant difference was observed between micafungin-treated and -untreated mice in both control and PF groups. Similar results were obtained in two different experiments and representative data are shown. Asterisks indicate p < 0.05 (Mann-Whitney U test). IFN-γ, interferon-gamma; IL-10, interleukin-10; IL-17A, interleukin-17A; IL-1β, interleukin-1 beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor alpha; PF, peritoneal fibrosis; and LPS, lipopolysaccharide.