Figure 3

FN treatment potently increased multinucleated myotube formation and its specific makers in the HSCG medium. (a) Multinucleated myotubes were captured by EVOS cell image system at 10X and 20X. (b) Protein lysates were prepared and used for immunoblotting using specific antibodies against key myogenic markers myoD, myogenin, myosin heavy chains (MHC) and α-tubulin. (c) The intensity of protein reacted bands was determined by densitometry using ImageJ software. Bars represent mean ± SEM for three replicates and statistical analysis was performed using a general linear model with multivariate, post hoc test and comparisons with respective controls. p < 0.05 level was considered significance between treatment and non-treatment.