Figure 4

Effects of FN on the expression of bone morphogenetic proteins (BMPs) in experimental cells. Cells were differentiated with HSCG medium in the presence/absence of different concentration of FN (1.25–5 µM) for 6 days. (a) BMPs mRNA expression in the experimental cells at different time points. (b) Levels of BMP-2 and BMP-4 protein expression in the experimental cells on day 6. Bars display mean ± SEM of six experimental replicates. Different letters a, b, c, and d within a column indicates significant differences between groups (p < 0.05). Statistical significance was performed using a general linear model with multivariate, post hoc test and comparisons with respective controls.