Figure 5 | Scientific Reports

Figure 5

From: Modulation of osteogenic and myogenic differentiation by a phytoestrogen formononetin via p38MAPK-dependent JAK-STAT and Smad-1/5/8 signaling pathways in mouse myogenic progenitor cells

Figure 5

FN role in JAKs-STATs and Smad1/5/8 signaling pathways in experimental cells. Cells were treated with FN in HSCG media for six days. Proteins were harvested and the phosphorylation levels of JAKs-STATs and Smad 1/5/8 proteins were analyzed on day 4 and 6 by immunoblotting using specific antibodies against targets. (a) JAKs-STATs and Smad 1/5/8 phosphorylation level after treatment with FN in HSCG medium. (b)The intensity of protein bands was quantified by densitometry using ImageJ software. Bars display mean ± SEM of three experimental replicates. *p < 0.05 represents a statistically significant difference between control and treatment.

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