Figure 6

Effect of FN on p38MAPK, AKT and p44/42 signaling pathways in experimental cells. Cells were treated with 2.5 μM FN in the presence of HSCG media for six days. Proteins were then extracted and analyzed by immunoblotting using specific antibodies against p38MAPK, AKT, and p44/42. (a) Regulation of p38MAPK, AKT, and p44/42 signaling by FN treatment. (b) The intensity of protein bands was determined by ImageJ software. Bars display mean ± SEM of three experimental replicates. *p < 0.05 indicates a statistically significant difference between treatment and non-treatment.