Table 3 Equilibrium dissociation constants (KD) for the interactions between [99mTc]Tc-labeled G3 variants and HER2-expressing SKOV3 cells. Experiments were performed in duplicates.

From: Optimal composition and position of histidine-containing tags improves biodistribution of 99mTc-labeled DARPin G3

Conjugate

KD1 (pM)

KD2 (nM)

[99mTc]Tc(CO)3-H6-G3

90 ± 5

3.3 ± 0.2

[99mTc]Tc(CO)3-G3-H6

118 ± 38

2.5 ± 1.5

[99mTc]Tc(CO)3-(HE)3-G3

96 ± 3

1.2 ± 0.1

[99mTc]Tc(CO)3-G3-(HE)3

89 ± 11

1.8 ± 0.5

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