Figure 2

Functional analysis of subchondral bone MSCs from medial and lateral condyles. (a) Comparison of MSC frequency measured as a proportion of colony forming units fibroblast in relation to total enzymatically-extracted cells for donor matched samples. (b) Phenotypic profile of culture expanded medial and lateral condyle derived MSCs indicating no differences in the expression of standard MSC markers. (c) Comparison of growth rates of medial and lateral condyle derived MSCs measured as population doubling times (in days) for donor matched samples. (d) Example images of differentiation assays performed with donor matched samples. Osteogenic images (Osteo) show positive alkaline phosphatase staining on day 14 post osteogenic induction, adipogenesis assay (Adipo) shows accumulation of Oil Red-O stained lipid vesicles on day 14 post adipogenic induction and toluidine blue staining of chondrogenic pellet cultures (Chondro) shows accumulation of proteoglycans (purple) on day 21 post chondrogenic induction. (e) Examples migration assay images from baseline (top) and 12 hours post scratch (bottom) wound for medial and lateral MSCs. Black line indicates the migrating front of the cells. (f) Analysis of migration assay images for donor matched medial and lateral MSCs showing the percentage of wound coverage by migrating cells after 12 hours (relative to the corresponding 0 hour area), and showing a trend for higher motility by lateral condyle MSCs.