Figure 4
The percentage loss of cholesterol from boar spermatozoa exposed to capacitating conditions as measured by the BODIPY-cholesterol assay (black bars) and mass spectrometric (MS) analysis (gray bars) for populations segregated based on viability. To isolate sperm populations, spermatozoa were stained with propidium iodide (PI) and either BODIPY-cholesterol labelled spermatozoa were gated for these different populations or cells were sorted based on these populations and then sperm lipids were analysed with mass spectrometry. Only in non-viable cells was there a major discrepancy between cholesterol efflux measured by BODIPY-cholesterol and lipid analysis. Results are based on six independent samples for both measures and presented as the mean percent cholesterol remaining ± SEM. *P < 0.05 indicate differences between cholesterol efflux measured by BODIPY-cholesterol and MS analysis for each sperm population.
