Figure 1

Membrane-coupled motors accumulate on static microtubule via membrane diffusion. (A) Cartoon schematic of motor protein clustering on a microtubule. (B–E) Fluorescence images of microtubule and GFP labeled kinesin clustering on an immobile microtubule recorded at 15, 45 and 75 minutes and (F–H) corresponding 1D line profiles of image intensity after background subtraction, corresponding to peaks of intensity. (I) Normalized GFP intensity as a function of time calculated from the average area under the peak for 45 microtubules. Error bars are calculated from the standard error of the mean, scale bar is 5 µm.