Table 3 Fecal microbiology of male Sprague-Dawley Conventional, SPF rats and SPF rats after 7 days acclimation in the laboratory prior to experimentation.

Bacteria and Fungi	Test Method	SPF Rats (AITHM Facility) (n = 2)	Laboratory Acclimated SPF Rats (n = 2)	Conventional Rats (Bush House) (n = 2)
Bordetella bronchiseptica	Culture	0/2	0/2	0/2
Bordetella spp	Culture	0/2	0/2	0/2
Corynebacterium kutscheri	Culture	0/2	0/2	0/2
Klebsiella oxytoca	Culture	0/2	0/2	0/2
^# Klebsiella pneumoniae	Culture	0/2	1/2^#	0/2
Other significant organisms	Culture	0/2	0/2	0/2
Pasteurella pneumotropica	Culture	0/2	0/2	0/2
Pasteurella spp	Culture	0/2	0/2	0/2
^{^} Proteus spp	Culture	2/2^	2/2^	0/2
Pseudomonas aeruginosa	Culture	0/2	0/2	0/2
Salmonella spp	Culture	0/2	0/2	0/2
Staphylococcus aureus	Culture	1/2	0/2	0/2
Streptococcus pneumoniae (alpha haem)	Culture	0/2	0/2	0/2
Streptococcus spp (beta haem)	Culture	0/2	0/2	0/2
Ectoparasite
Mycoptes musculinis	Exam	0/2	0/2	0/2
Myobia musculi	Exam	0/2	0/2	0/2
Polyplax spinulosa	Exam	0/2	0/2	0/2
Radfordia spp	Exam	0/2	0/2	0/2
Endoparasite
Giardia muris	Wet prep/Motility	0/2	0/2	0/2
Pinworm – Aspiculuris spp	Wet prep/Motility	0/2	0/2	0/2
Pinworm – Aspiculuris tetraptera	PCR	0/2	0/2	0/2
Pinworm – Syphacia muris	PCR	0/2	0/2	0/2
Pinworm – Syphacia spp	Wet prep/Motility	0/2	0/2	0/2
Spironucleus muris	Wet prep/Motility	0/2	0/2	0/2
Non-Pathogenic Protozoa
Chilomastix bettencourti	Wet prep/Motility	0/2	0/2	0/2
*Entamoeba muris	Wet prep/Motility	1/2*	1/2*	1/2*
Tritrichomonas muris	Wet prep/Motility	0/2	0/2	0/2

Microbiology results obtained from Cerberus Sciences on two SPF animals, two SPF animals acclimated for seven days in a conventional facility, and two conventional animals. Results presented as number of positive samples per number tested (2).
^#Klebsiella pneumoniae has been associated with bacteraemic disease and widespread abscesses. They inhabit the gastrointestinal tract and are opportunistic agents waiting for the correct conditions to cause infections.
^{^}Proteus species are gram-negative, rod-shaped, and facultatively anaerobes. They are ubiquitous in the environment and can be cultured from the upper respiratory tract and feces. They are commonly the cause of urinary tract infections in immunocompromised animals. The most common species is Proteus mirabilis.
^*Entamoeba muris are considered non-pathogenic and frequently found in intestinal contents of normal rodents.

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