Figure 7

Cluster of species with similar evolutionary relationship based on Principal Co-ordinates Analysis (n = 4 per group, PCoA) (A). Relative abundance of the most abundant bacterial at genus level (B), the analysis of Ruminococcus genus based on RMG and NCBI database were displayed as g_-Ruminococcus- and g_Ruminococcus respectively. The taxonomic cladogram obtained from LEfSe analysis of 16S sequences (relative abundance ≥0.1%) (C). Linear Discriminant Analysis (LDA) based value compared between group CK, O157 and PR39 + O157 (D). The length of the histogram represented the effects size of difference bacteria and the higher score of LDA of the bacteria meaned the more dominant in the sample. Only taxa meeting an LDA scoreå 2 and P <0.05 are shown. The counts of E. coli in fecal per gram contents during each day (E). The colonies of E. coli in liver (F) and spleen (n = 6 per group) (G). The concentrations of acetate (H), propionate (I) and butyrate (J) in the cecal contents per gram determined by gas chromatography (n = 6 per group). *Means p < 0.05, **means p < 0.01, ***means p < 0.001.