Figure 4

Evaluation of mitochondria network and mitophagic-vacuoles. (A) Representative images of ATP5C1-Rosella reporter. Mitochondria network mask on green perimeter and mitophagic-vacuole mask on red perimeter. Scale bars indicate 20 µm. (B) Image analysis workflow for mitophagy with ATP5C1-Rosella reporter line. Scale bars indicate 20 µm and 3x zoomed insets are highlighted with yellow boxes. Contrast of displayed images was adjusted by saturating the bottom and the top 1% of all the pixels values. (C) Mitochondrial and mitophagy volumes in basal conditions. (D) Mitophagy frequencies in basal condition and after mitochondrial stress induction. (E) Evaluation of mitophagy levels and distribution of autophagy resources upon mitochondrial stress. Percentages for mitophagy and non-mitochondrial autophagy are indicated.