Figure 1
Characterization of CLI mouse model and skeletal muscle regeneration. (a) LDPI of ventral mouse hindlimbs 1 hour, 3 days, 7 days, 14 days, 28 days, and 56 days following CLI. Control leg on left, ischemic leg on right. Scale bar represents blood flow perfusion by color. (b) H&E staining of TA cross-sections in control, and 3 days, 7 days, 14 days, 28 days, and 56 days following CLI. (c) Immunohistochemistry of TA cross-sections in control, and 3 days, 7 days, 14 days, 28 days, and 56 days following CLI. Dystrophin pseudo-colored in red, eMHC in green, nuclei in blue. Scale bars on cross-sections represent 50 µm. (d) Total number of eMHC+ fibers within a 0.33 mm2 field of view for control, 7 days, 14 days, and 56 days following CLI. (e) Percentage of centrally nucleated fibers in control, 14 days, 28 days, and 56 days following surgery. (f) Mean cross-sectional fiber area of 4 random 0.33 mm2 fields of view of the TA using dystrophin in control, 14 days, 28 days, and 56 days following CLI. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to control for all figures. (g) Mean cross-sectional area of centrally nucleated myofibers of the TA. n = 3, ****p < 0.0001 compared to control. ##p < 0.01, ###p < 0.001 compared to day 56.
