Figure 6
Impaired mitochondrial function following CLI. (a) Basal (state 1 respiration) oxygen consumption rate and mitochondrial hydrogen peroxide (H2O2) production from hindlimb skeletal muscles 7 days following CLI using Oroboros Oxygraph-2k (n = 3). (b) Quantified Western blot analyses 7, 14, and 28 days following CLI for mitochondrial ETC complex I (NDUFB8-subunit), complex II (SDHB-subunit), complex III (UQCRC2-subunit), complex IV (MTCO1-subunit), and complex V (ATP5A-subunit) relative protein expression (n = 3). (c) Quantified Western blot analyses 14 days following CLI for SOD2 (n = 3), SOD1 (n = 6), and myoglobin (n = 6) protein expression. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to control for all figures.
