Figure 5
Mutant BK β1 channel expression in HEK 293 cells. (a) Immunofluorescence of HEK cells showing plasma membrane expression of β1 mutant W163I expressed alone, (b) or in combination with α subunit. (c) Immunofluorescence of β1 mutant F166A expressed alone, (d) β1 mutant F159A (e) β1 mutant T169A and (f) of β1 mutant L172A. (g) Quantification of levels of membrane expression measured by flow cytometry (% cells), β1W163I: 43.21 ± 4.1 (blue bar); β1, α/β1W163I: 31.71 ± 2.4 (blue and black squared pattern) using an antibody against β1 when α/β1W163I were co-transfected; β1F166A: 21.74 ± 1.9% (green bar); β1F159A: 21.10 ± 0.3% (purple bar); β1T169A: 23.98 ± 1.1 (orange bar); β1L172A: 35.63 ± 1.1 (yellow bar); antibody against α when α/β1W163I were co-transfected (blue and white squared pattern bar): 32.59 ± 2.5; Error bars: SEM. Red: anti-MaxiK-β1and Alexa Fluor 568 antibodies; blue: DAPI nuclear stain.
