Figure 8
E2 is unable to modulate VSD equilibrium in α/β1W163I and α/β1F166A mutant channels. (a) Left: representative gating current recordings from α/β1W163I channels (blue traces), in the absence or presence of E2, as indicated. Right: Q/Qmax versus membrane potential relationships forα/β1W163I in control (filled blue circles) and after the addition of E2 to a final concentration of 10 μM (open blue circles). n = 3. (b) Left: representative gating current recordings from α/β1F166A channels (green traces), in the absence or presence of E2, as indicated. Right: Q/Qmax versus membrane potential curves for α/β1F166A in control (filled green circles) and after the addition of E2 to a final concentration of 10 μM (open green circles), n = 3. The red line corresponds to the Boltzmann function that best fit the α/β1 channel data.
