Figure 1

An overview of the study outline. At postnatal day 14 (P14), the animals were subjected to a single dose of 8 Gy cranial irradiation (CIR). The animals were sacrificed 4 months later. One group of animals was used for immunohistochemistry (IHC) to quantify neurons (NeuroTrace^TM), Iba1⁺ microglia, Olig2⁺ oligodendrocytes and S100⁺ astrocytes in the thalamus. Another group of animals was used to isolate S100β⁺ thalamic astrocytes using fluorescence-activated cell sorting (FACS) and perform gene expression profiling using next-generation sequencing (NGS).