Figure 4

FACS analysis of S100β⁺ astrocytes in the brain following CIR. Gating strategy for sorting of S100β⁺ astrocytes from the thalamus. (A) shows the SSC vs FSC plot which was used to gate cells from debris, (B) shows a negative control staining where the primary antibody was omitted and the (C) panel shows a representative sample stained with S100β. (D) show quantification of the relative numbers (%) of S100β⁺ astrocytes within the entire heterogeneous cell population analyzed by flow cytometry during the isolation of astrocytes with FACS. ***p < 0.001. Data shown as mean ± SEM. CIR = Cranial irradiation, FACS = fluorescence-activated cell sorting, FSC = forward scatter, SSC = side scatter, n_control = 20 and n_irradiated = 12.