Figure 5
Staining of cell wall chitin and lipid content in the different morphotypes of Zymoseptoria tritici. Cell walls were stained with the chitin-binding dye Calcofluor White (blue color) and the lipid droplets were stained with Nile Red (red color). Blastospores, hyphae, and pseudohyphae showed a weaker fluorescence for Calcofluor White compared to free chlamydospores and those formed terminally or intercalarily via suspensor cells. A high accumulation of lipid droplets was observed in chlamydospores, as well as in blastospores and pseudohyphae. Hyphae only showed a high accumulation of lipid droplets in the hyphal branching zone. Bars represent 10 μm.
