Figure 7
Analysis of read-through transcripts traversing the sdsR terminator. (A) Schematic representation of sdsR-tRNAArg-CAT fusion plasmids. The B. albidum tRNAArg gene was inserted in the region before the CAT gene of pKK232-8 plasmid. (B) Northern blot analysis of Arg-tRNA, RyeA, and SdsR. Total cellular RNA from cells carrying the sdsR(−379/+222)-tRNAArg-CAT or sdsR(−379/+222ryeAPm)-tRNAArg-CAT fusion plasmid were isolated and analyzed by Northern blotting. In each panel the spliced images from the same Northern membrane are shown with the insertion of a dividing lines between the spliced lanes.
