Figure 3
From: An Innovative Platform Merging Elemental Analysis and Ftir Imaging for Breast Tissue Analysis

Illustration of the process followed for background subtraction. A. the rectangles represent the areas selected to be used as background in this 13C image. In this example, 1918 spectra were included in the rectangles and their mean was subtracted from all spectra. B. intensity distribution before subtraction of the background, C. intensity distribution after subtraction of the background, D. intensity distribution after subtraction of the mean and division by the standard deviation.