Figure 1 | Scientific Reports

Figure 1

From: Dual usage of a stage-specific fluorescent reporter system based on a helper-dependent adenoviral vector to visualize osteogenic differentiation

Figure 1

The structure of the HDAd-hOC-Venus vector. (a) A 2.6-kb PCR fragment of the Venus-pA-FRT-PGK-EM7-neo-pA-FRT cassette was amplified using PCR primers with 40-nt homology to the OC locus. ATG: translation initiation codon. NeoR: neomycin-resistant gene cassette. Red triangles: FRT sites. (b) Human OC locus. VDRE: vitamin D receptor regulatory element. (c) The human OC locus with the insertion of the Venus gene by Red/ET homologous recombination. (d) Linearized pHDAd-hOC-Venus vector constructed by subcloning a total of 21.4 kb of homologies, including the marker cassette, into pAMHDAdGT8-4. Orange bars: 5′ and 3′ human OC gene homology arms. TK: herpes simplex virus thymidine kinase gene. LacZ: E. coli β-galactosidase gene. Black triangles: adenoviral inverted terminal repeat (ITR). (e) HDAd-hOC-Venus vector packaged as virus.

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