Figure 3 | Scientific Reports

Figure 3

From: Dual usage of a stage-specific fluorescent reporter system based on a helper-dependent adenoviral vector to visualize osteogenic differentiation

Figure 3

The specific expression of Venus at bone-like nodules in human induced osteoblasts from the OC-Venus knock-in hiPSC line. (a) The timeline of human osteoblast induction. Each sampling time is indicated as (b–d) for MSCs and (e–g) osteoblasts, respectively. MSCs and osteoblasts induced from control and OC-Venus knock-in human iPSC lines were observed live under an IX81 fluorescence microscope, using a 10x objective lens. Ph: phase contrast images obtained with an exposure time of 10 ms in grayscale. Venus: GFP filter images obtained with an exposure time of 500 ms in green. Scale bar: 100 μm. (b) MSCs without osteoblast induction derived from the control (CT; TIG3/KOSM) line. (c) MSCs without osteoblast induction from the OC-Venus knock-in (EX #11; OCV37FF11) iPSC line. (d) MSCs from (a,b) were dissociated and analyzed using a FACS Area III. The black line indicates the FACS histogram of CT and the green line indicates that of EX #11. (e) Osteoblasts derived from CT on day 31. (f) Images of living osteoblasts from EX #11 at day 31. (g) Osteoblasts from (d,e) were dissociated and analyzed using a FACS Area III. The black line indicates the FACS histogram of CT and the green line indicates that of EX #11.

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