Figure 5 | Scientific Reports

Figure 5

From: Dual usage of a stage-specific fluorescent reporter system based on a helper-dependent adenoviral vector to visualize osteogenic differentiation

Figure 5

Mouse primary osteoblasts infected with HDAd-hOC-Venus and E1DAd-hOC3.8-Venus. Mouse POBs were cultured for 6 days and then infected with either HDAd-hOC-Venus, E1DAd-hOC3.8-Venus, or E1DAd-CMV-GFP at an MOI of 1,000. Three days later, the cells were stained with an anti-OC antibody, and observed under an IX81 fluorescence microscope using a 20x objective lenses. Ph: phase contrast images obtained with an exposure time of 10 ms in grayscale. Merge: GFP filter image in green, Anti-OC image in red, DAPI image in blue. All of the fluorescence images were obtained with an exposure time of 1 s. Scale bar: 100 μm. FACS: FACS scatterplots of the primary osteoblasts infected with each AdV vector and without immunostaining were created using a FACS Calibur. The X- and Y-axes show hOC-Venus (Venus) and autofluorescence (FL2-H), respectively.

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