Table 1 Constitutive expression changes in AMPK subunit genes between TF, TR and WT coho salmon groups

AMPK subunit gene	P-value	TF mean ± s.d.	TR mean ± s.d.	WT mean ± s.d.
AMPK-α1A1	0.082	0.56 ± 0.04^A	0.71 ± 0.13^A	0.55 ± 0.14^A
AMPK-α1A2	0.049	1.03 ± 0.23^A	1.02 ± 0.17^A	0.70 ± 0.23^A
AMPK-α2A1	0.021	2.38 ± 0.49^A	1.90 ± 0.36^A,B	1.38 ± 0.57 ^B
AMPK-α2A2^	0.031	7.16 ± 3.79^A,B	7.53 ± 1.97^A	4.00 ± 1.14^B
AMPK-β1A	0.003	3.81 ± 0.52^B	5.62 ± 0.96^A	3.01 ± 1.23^B
AMPK-β1B	0.001	0.52 ± 0.07^A	0.48 ± 0.14^A	0.25 ± 0.05^B
AMPK-β2A1	0.478	16.59 ± 1.93^A	20.98 ± 4.52^A	17.55 ± 8.79^A
AMPK-β2A2	0.284	2.30 ± 0.31^A	2.61 ± 0.56^A	2.08 ± 0.61^A
AMPK-γ1A1	0.012	0.41 ± 0.06^A,B	0.54 ± 0.15^A	0.30 ± 0.08^B
AMPK-γ1A2^	0.325	0.09 ± 0.02^A	0.17 ± 0.10^A	0.11 ± 0.03^A
AMPK-γ2A1^	0.071	0.50 ± 0.27^A	0.41 ± 0.14^A	0.24 ± 0.09^A
AMPK-γ2A2^	0.070	0.12 ± 0.04^A	0.33 ± 0.31^A	0.12 ± 0.05^A
AMPK-γ2B1^	<0.001	7.82 ± 2.51^A	1.96 ± 0.76^B	1.79 ± 0.82^B
AMPK-γ2B2^	0.002	21.84 ± 11.69^A	8.54 ± 1.38^A,B	4.38 ± 2.71^B
AMPK-γ3A*	0.011	1.94 ± 0.45^B	3.57 ± 0.2^A	3.66 ± 0.5^A

Transcript levels (mean ± s.d., n = 5) are given for the three experiment groups (PBS-injected, controls) and are quantitatively comparable across groups/genes. For each gene, different superscript letters indicate significant differences across groups. ^{‘^’}Highlights genes that required Box-Cox transformation. ^‘*’Highlights genes where a Kruskal-Wallis test was used.

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