Figure 2
From: Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms
Down-regulation of prophage genes in the C. difficile luxS mutant. (A) Pairwise analysis identified 21 differentially expressed genes in luxS (red points). All 18 down-regulated genes clustered into two regions. (B) Three prophage regions are identified in the C. difficile genome using Phaster. Regions 2 and 3 were down regulated in luxS. (C) Heat map representation of the genes that were differentially expressed in luxS, red and green indicate down- and up-regulation respectively when compared to WT. 18 prophage genes were found to be down-regulated in luxS relative to WT, whilst two genes involved in trehalose metabolism were up-regulated in luxS relative to WT. Data shown is the mean of 3 independent experiments in triplicates. Differential expression was defined as ≥1.6-fold change relative to WT with an adjusted p-value ≤ 0.05.
