Figure 5
From: Bacterial Density and Biofilm Structure Determined by Optical Coherence Tomography
Signal intensity analysis of OCT images: biofilms of non-EPS producing S. epidermidis 252 and EPS producing S. epidermidis ATCC 35984. (A) Schematic presentation of non-EPS producing S. epidermidis 252 and EPS producing S. epidermidis ATCC 35984 biofilms (see Table 1) and their measured thickness (derived using Otsu thresholding of auto-scaled OCT images) and volumetric bacterial density, calculated from the biofilm thickness and enumeration of the number of bacteria in the biofilm. (B) Intensity as a function of biofilm height (%) for S. epidermidis 252 and S. epidermidis ATCC 35984 biofilms in auto-scaling analysis. (C) Same as (B), but now in re-scaling analysis. Error bars indicate standard deviations over triplicate experiments with separate bacterial cultures.
