Figure 4
From: Engineering multi-layered tissue constructs using acoustic levitation
The effect of an E-cadherin neutralising antibody on formation of stable epithelial cell sheets. A single cell suspension of 16HBE cells was introduced into the acoustic bioreactor in the presence of a neutralising antibody to E-cadherin or isotype control and the size of the agglomerate area determined every 10 min for 6 h. Data are mean ± SD (a) or representative phase-contrast photomicrographs of the cell sheets after removal from the bioreactor following levitation for 6 h (b), n = 3. Scale bar is 250 μm. **P ≤ 0.01, ***P ≤ 0.001 compared to 0 h and +++P ≤ 0.001 compared to E-cadherin neutralising antibody (two-way ANOVA with Sidak’s correction for multiple comparisons).
