Figure 1

Heat map, Principal component analysis and Hierarchical clustering dendrogram in IDH mutant/normal gliomas. (A) The clustered heat map shows 396 biochemicals in lysates from five replicates each of IDH mutant glioma tissues (Pt1–Pt5) and normal IDH tissues (Pt6–Pt10) (Left). The heat map for U87 glioblastoma cells shows 261 biochemicals in triplicate lysates from cells expressing IDH1 mutation (m1–m3), normal IDH1 (n1–n3), or vector alone (v1–v3) (Right). (B) 3D plot of principal component analysis (PC Analysis) with fitted ellipses for the biochemicals in the major class category of The Human Metabolome Database (HMDB 3.0) (151 and 115 biochemicals in glioma tissues and U87 cells, respectively). PC1, PC3 and PC4 for glioma tissues (left, PC2 was omitted from the analysis as it did not show any relevant clustering information), and PC1, PC2 and PC3 for U87 cells (right) were plotted. In each group of glioma tissues, 95% confidence interval ellipsoid was illustrated. (C) Hierarchical clustering dendrogram obtained from the three principal components described in B.